DPBS Calculator Without Calcium or Magnesium
Scale a defined Dulbecco’s phosphate-buffered saline formulation without calcium or magnesium at pH 7.2.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Sodium chlorideanhydrous | 8 g | |
| Potassium chlorideanhydrous | 200 mg | |
| Sodium phosphate dibasicanhydrous | 200 mg | |
| Potassium phosphate monobasicanhydrous | 200 mg | |
| Purified water | Bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
Verify the final measured pH experimentally.
How to prepare DPBS without calcium and magnesium, pH 7.2
- Add the calculated NaCl, KCl, Na₂HPO₄, and KH₂PO₄ to approximately 70–80% of the selected final volume of purified water.
- Stir until all salts dissolve completely.
- Bring to the selected final volume with purified water.
- Mix thoroughly and verify the final measured pH experimentally.
About This Buffer
Dulbecco’s phosphate-buffered saline (DPBS) is a phosphate-buffered saline formulation commonly used in cell-culture workflows for washing, dilution, and sample handling. DPBS is available in multiple formulations, including versions with or without calcium and magnesium. The ChemH calculator specifically provides a calcium- and magnesium-free 1X formulation with an expected pH of 7.2.
Common questions
Does this DPBS contain calcium or magnesium?
No. This reviewed preset is explicitly calcium- and magnesium-free.
How is DPBS different from the PBS page?
This page scales one defined DPBS formulation at pH 7.2, while the PBS page contains separate 1X and 10X PBS recipes at pH 7.4.
Can calcium or magnesium be added with this calculator?
No. A calcium- or magnesium-containing DPBS formulation would require a separate complete and reviewed preset.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
DPBS without calcium and magnesium, pH 7.2
Source location: Solutions — exact DPBS without calcium and magnesium, pH 7.2 formulation.
Preparation method: Source-stated quantitative composition
- Assessment and Imaging of Intracellular Magnesium in SaOS-2 Osteosarcoma Cells PMC8073505 · open article