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How to Prepare 1 M Tris-HCl Buffer (pH 8.0)
Introduction Tris-HCl is a common buffer in molecular biology and biochemistry. It is used in protein, nucleic acid, electrophoresis, and enzyme workflows. Tris, or tris(hydroxymethyl)aminomethane, is a weak base. Its useful buffering range is about pH 7.0–9.0. This makes it…