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How to Prepare 1 M Tris-HCl Buffer (pH 8.0)

1 M Tris-HCl Buffer

Introduction Tris-HCl is a common buffer in molecular biology and biochemistry. It is used in protein, nucleic acid, electrophoresis, and enzyme workflows. Tris, or tris(hydroxymethyl)aminomethane, is a weak base. Its useful buffering range is about pH 7.0–9.0. This makes it…

How to Make Phosphate Buffered Saline (PBS)

Phosphate Buffered Saline (PBS)

What is Phosphate Buffered Saline? Phosphate buffered saline (PBS) is a special saltwater solution used in science labs. It is a buffered solution, which means it can keep its pH (acidity) steady. The pH of PBS is about 7.4, similar…