Ammonium Bicarbonate Buffer Calculator
Scale fresh 50 mM or 100 mM ammonium bicarbonate preparations with a nominal pH of approximately 7.8.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Ammonium bicarbonatesolid | 197.6 mg | |
| Purified water | Bring the total solution volume to 50 mL. Do not add 50 mL of water; add water only until the total solution reaches 50 mL. |
How to prepare 50 mM ammonium bicarbonate
- Select the 50 mM or 100 mM preset and add the calculated ammonium bicarbonate to less than the selected final volume of purified water.
- Stir until the solid dissolves completely.
- Bring to the selected final volume and mix thoroughly.
- Treat pH 7.8 as nominal rather than an adjusted target, and prepare the solution fresh.
About This Buffer
Ammonium bicarbonate is a volatile buffering system widely used in protein digestion and mass-spectrometry sample-preparation workflows. Concentrations in the tens of millimolar range, including 50–100 mM, are commonly used in proteomic procedures. This calculator scales fresh 50 mM and 100 mM ammonium bicarbonate preparations with a nominal pH of approximately 7.8 rather than an experimentally adjusted pH target.
Common questions
What concentrations are available?
The page provides separate fixed presets for 50 mM and 100 mM ammonium bicarbonate.
Is the pH adjusted to exactly 7.8?
No. The recipe reports an expected or nominal pH of approximately 7.8 and does not include a titration step.
Why does the workflow say to prepare the buffer fresh?
The reviewed recipe explicitly identifies ammonium bicarbonate as a fresh preparation, so the page should preserve that instruction without inventing a shelf life.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
50 mM ammonium bicarbonate
Source location: Reagents — fresh 50 mM ammonium bicarbonate buffer.
Preparation method: Source-stated quantitative composition
- Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2 PMC10062472 · open article
View sources for all presets
100 mM ammonium bicarbonate
Source location: Reagents — fresh 100 mM ammonium bicarbonate buffer.
Preparation method: Source-stated quantitative composition
- Proximity Protein Labeling In Dictyostelium With Engineered Ascorbic Acid Peroxidase 2 PMC10062472 · open article