Triton X-100 Lysis Buffer Calculator
Scale a defined Tris/NaCl/EDTA lysis buffer containing 1% Triton X-100 at pH 7.4.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| 1 M Tris-HCl pH 7.4prepared aqueous stock | 5 mL | |
| 5 M sodium chlorideprepared aqueous stock | 3 mL | |
| 0.5 M EDTA solution, pH 8.0prepared aqueous stock | 200 µL | |
| 20% Triton X-100prepared aqueous stock | 5 mL | |
| Purified water | Bring the total solution volume to 100 mL. Do not add 100 mL of water; add water only until the total solution reaches 100 mL. |
How to prepare Triton lysis buffer pH 7.4
- Add the calculated 1 M Tris-HCl pH 7.4, 5 M NaCl, and 0.5 M EDTA solution, pH 8.0 volumes to less than the selected final volume of purified water.
- Add the calculated 20% Triton X-100 stock volume slowly while mixing gently.
- Bring to the selected final volume with purified water.
- Mix until uniform while avoiding excessive foaming.
- Add the protease inhibitor fresh before use when required by the extraction method.
About This Buffer
Triton X-100 lysis buffer uses a nonionic detergent to solubilize membrane components and extract proteins under conditions that differ from multi-detergent RIPA formulations. This page scales a defined formulation containing 50 mM Tris, 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100 at pH 7.4. It does not contain the deoxycholate or SDS present in the ChemH RIPA preset. A protease inhibitor used in the cited extraction method is method-specific and is not part of the default scalable buffer calculation.
Common questions
What is the final composition of this Triton lysis buffer?
The preset contains 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, and 1% Triton X-100.
Why does the recipe use a 20% Triton X-100 stock?
The reviewed preparation defines Triton X-100 as a stock solution, allowing its volume to be scaled consistently with the other components.
How is this formulation different from RIPA?
This buffer contains Tris, NaCl, EDTA, and Triton X-100 but does not include the deoxycholate, SDS, or EGTA components present in the RIPA preset.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
Triton lysis buffer pH 7.4
Source location: Reagents — Triton X-100 lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100). The source-defined EDTA stock is represented without inferring a salt or hydration state. When following the cited extraction method, add the source-specified protease inhibitor fresh before use; it is method-specific and is not part of the default scalable buffer calculation.
Preparation method: Fixed mixing ratio of defined stock solutions