1 M Bicine Buffer Calculator
Scale separate 1 M Bicine-NaOH stock recipes at pH 8.0 or 8.5 while preserving each reviewed preset as an independent formulation.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Bicinefree compound | 16.32 g | |
| Sodium hydroxide | Adjust experimentally to pH 8.0. | |
| Purified water | After pH adjustment, bring the total solution volume to 100 mL. Do not add 100 mL of water; add water only until the total solution reaches 100 mL. |
How to prepare 1 M Bicine-NaOH pH 8.0
- Select pH 8.0 or pH 8.5 and add the calculated Bicine to approximately 70–80% of the selected final volume of purified water.
- Stir until the Bicine dissolves completely.
- Add NaOH gradually until the selected target pH is reached.
- Bring to the selected final volume, mix thoroughly, and verify the final pH.
About This Buffer
Bicine is a zwitterionic biological buffer suitable for biochemical work under mildly alkaline conditions. This calculator provides separate 1 M Bicine stock formulations at pH 8.0 and 8.5. Both use the same Bicine concentration, while the selected final pH is established experimentally with NaOH.
Common questions
Why do the pH 8.0 and pH 8.5 presets use the same Bicine mass?
Both presets are 1 M Bicine, so the Bicine amount per final volume is the same. The difference is the experimentally adjusted pH.
Why are the two pH values separate presets?
Each pH value is tied to its own reviewed preparation record. The calculator does not interpolate between pH values.
What Bicine molecular weight is used?
The mass basis uses Bicine with a molecular weight of 163.17 g/mol.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
1 M Bicine-NaOH pH 8.0
Source location: Reagents — exact 1 M Bicine pH 8.0 preparation; PubChem identity cross-check.
Preparation method: Source-stated quantitative composition
- Quantitative Analysis of Redox Pool in Plant Samples Under Aluminum Stress PMC9257835 · open article
- PubChem Compound Summary for Bicine (CID 8761) PUBCHEM_BICINE · public authority
View sources for all presets
1 M Bicine-NaOH pH 8.5
Source location: Preparation — 1 M Bicine adjusted to pH 8.5; independent use; PubChem molecular weight.
Preparation method: Mass derived from the published concentration
- Increasing the Compositional Heterogeneity of Single-Chain Amphiphile Membranes Supported by Coacervate Cores Alters Stability and Properties of the Hybrid Protocells PMC13192312 · open article
- Measuring Vesicle Loading with Holographic Microscopy and Bulk Light Scattering PMC11274288 · open article
- PubChem Compound Summary for Bicine (CID 8761) PUBCHEM_BICINE · public authority