Carbonate-Bicarbonate ELISA Coating Buffer Calculator
Scale a fixed 50 mM carbonate-bicarbonate ELISA coating buffer at pH 9.6 from sodium carbonate and sodium bicarbonate.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Sodium carbonateanhydrous | 1.59 g | |
| Sodium bicarbonateanhydrous | 2.93 g | |
| Hydrochloric acid or sodium hydroxide | Measure the pH and adjust experimentally to pH 9.6 using HCl or NaOH as required by the measured pH. Do not calculate or predict the titrant amount. | |
| Purified water | After pH adjustment, bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
How to prepare 50 mM carbonate-bicarbonate pH 9.6
- Add the calculated anhydrous sodium carbonate and sodium bicarbonate to approximately 70–80% of the selected final volume of purified water.
- Stir until both solids dissolve completely.
- Measure the pH and adjust experimentally to pH 9.6 with HCl or NaOH as required by the measured pH.
- Bring to the selected final volume with purified water, mix thoroughly, and verify the final pH.
About This Buffer
Carbonate-bicarbonate buffer at alkaline pH is commonly used as a coating buffer in ELISA and related immunoassays. The alkaline buffer provides conditions commonly used for passive adsorption of proteins or antibodies to assay plates. This calculator scales one fixed 50 mM carbonate-bicarbonate formulation and adjusts the final mixture experimentally to pH 9.6 with HCl or NaOH as required by the measured pH.
Common questions
What is the fixed concentration of this ELISA coating buffer?
The reviewed preset is a 50 mM carbonate-bicarbonate formulation at pH 9.6.
Why does the recipe contain both sodium carbonate and sodium bicarbonate?
The fixed amounts of the two components define the reviewed carbonate-bicarbonate formulation and should be scaled together without changing their ratio.
Should I verify the pH after preparation?
Yes. Adjust experimentally to pH 9.6 with HCl or NaOH as required by the measured pH, without predicting a titrant amount, then verify the final pH.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
50 mM carbonate-bicarbonate pH 9.6
Source location: Recipes, 50 mM Carb/Bicarb Buffer — dissolve 1.59 g Na2CO3 and 2.93 g NaHCO3 in 1 L deionized water, then adjust to pH 9.6 using HCl or NaOH.
Preparation method: Source-stated quantitative composition
- Assay for Assessing Mucin Binding to Bacteria and Bacterial Proteins PMC8005912 · open article