HEPES-Buffered Saline Calculator
Scale a defined HEPES-buffered saline containing 10 mM HEPES and 150 mM sodium chloride at pH 7.4.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| HEPES free acidfree acid | 2.383 gMass derived from 0.010 mol/L × 1 L × 238.30 g/mol. | |
| Sodium chlorideanhydrous | 8.766 g | |
| pH adjustment reagent (unspecified) | Measure and adjust to pH 7.4. The registered composition source does not identify the adjustment reagent/titrant, so none is inferred. | |
| Purified water | After pH adjustment, bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
How to prepare HBS pH 7.4
- Add the calculated HEPES free acid and NaCl to approximately 70–80% of the selected final volume of purified water.
- Stir until both components dissolve completely.
- Measure and adjust to pH 7.4.
- Bring to the selected final volume with purified water.
- Mix thoroughly and verify the final pH.
About This Buffer
HEPES-buffered saline (HBS) combines HEPES with sodium chloride to provide buffering and ionic strength. Because HBS compositions vary among protocols, this page scales one explicitly defined formulation containing 10 mM HEPES and 150 mM NaCl at pH 7.4. The final mixture is adjusted experimentally to pH 7.4, but the reviewed composition source does not identify the titrant.
Common questions
What is the final composition of this HBS preset?
The reviewed formulation contains 10 mM HEPES and 150 mM NaCl at pH 7.4.
Does this HBS recipe use HEPES free acid?
Yes. The mass is derived from 10 mM HEPES free acid using the registered molecular weight of 238.30 g/mol. The registered composition source does not identify the titrant, so the page does not name one.
Is every buffer called HBS compositionally identical?
No. HEPES-buffered saline can refer to multiple formulations. This page scales one explicitly defined recipe.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
HBS pH 7.4
Source location: Methods — HBS final composition (10 mM HEPES, 150 mM NaCl, pH 7.4); PubChem HEPES molecular weight. The registered composition source does not identify the adjustment reagent/titrant.
Preparation method: Mass derived from the published concentration
- Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies PMC2669081 · open article
- PubChem Compound Summary for HEPES (CID 23831) PUBCHEM_HEPES · public authority