10X MOPS RNA Electrophoresis Buffer Calculator
Scale a 10X MOPS RNA electrophoresis buffer at pH 7.0 using MOPS, sodium acetate trihydrate, and a source-defined 0.5 M EDTA solution at pH 8.0.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| MOPS free acidfree acid | 83.7 g | |
| Sodium acetate trihydratetrihydrate | 13.6 g | |
| 0.5 M EDTA solution, pH 8.0prepared aqueous stock | 20 mL | |
| Sodium hydroxide | Adjust experimentally to pH 7.0. | |
| Purified water | After pH adjustment, bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
How to prepare 10X MOPS RNA buffer pH 7.0
- Add the calculated MOPS free acid and sodium acetate trihydrate to approximately 70–80% of the selected final volume of purified water.
- Stir until the solids dissolve, then add the calculated volume of 0.5 M EDTA solution, pH 8.0 and mix thoroughly.
- Add NaOH gradually while monitoring the pH until pH 7.0 is reached.
- Bring to the selected final volume with purified water.
- Mix thoroughly and verify the final pH.
About This Buffer
MOPS-based buffer is commonly used in denaturing agarose-formaldehyde electrophoresis of RNA. The ChemH formulation contains MOPS, sodium acetate trihydrate, and EDTA and is prepared as a 10X stock at pH 7.0. The final pH is established experimentally with NaOH, and this formulation is intended for RNA electrophoresis rather than as a general-purpose MOPS stock.
Common questions
How is EDTA represented in this MOPS recipe?
The source-defined 0.01 M final EDTA concentration is implemented as 20 mL of prepared 0.5 M EDTA solution, pH 8.0 per liter. No EDTA salt or hydration state is inferred.
How do I prepare 1X MOPS RNA buffer from 10X stock?
Use a 1:10 dilution of the completed 10X stock when the electrophoresis protocol calls for 1X buffer.
Why is the NaOH volume not calculated?
NaOH is used experimentally to reach pH 7.0 and is not treated as a scalable quantitative component.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
10X MOPS RNA buffer pH 7.0
Source location: Thermo Fisher protocol — 10X MOPS contains 0.4 M MOPS, 0.1 M sodium acetate, and 0.01 M EDTA at pH 7.0, implemented with 20 mL of source-defined 0.5 M EDTA solution, pH 8.0 per liter; the EDTA salt and hydration state are not inferred. PMC4126239 provides an independent 10X MOPS preparation with NaOH adjustment, and PMC8325231 documents use in formaldehyde-gel electrophoresis.
Preparation method: Source-defined final concentrations implemented with calculated volumes of defined stocks
- Denaturing Formaldehyde Gels in MOPS Buffer THERMO_MOPS_FORMALDEHYDE_GELS · manufacturer protocol
- Optimized RNA ISH, RNA FISH and protein-RNA double labeling (IF/FISH) in Drosophila ovaries PMC4126239 · open article
- Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot PMC8325231 · open article