0.5 M PIPES Buffer Calculator, pH 6.8
Scale a reviewed 0.5 M PIPES free-acid stock recipe at pH 6.8 and calculate the required PIPES mass for the selected final volume.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| PIPES free acidfree acid | 75.6 g | |
| Sodium hydroxide | Adjust experimentally to pH 6.8. | |
| Purified water | After pH adjustment, bring the total solution volume to 500 mL. Do not add 500 mL of water; add water only until the total solution reaches 500 mL. |
How to prepare 0.5 M PIPES-NaOH pH 6.8
- Add the calculated PIPES free acid to approximately 70–80% of the selected final volume of purified water.
- Stir to form a uniform suspension. Do not require complete dissolution before adding NaOH.
- Add NaOH gradually while mixing. Continue until the PIPES dissolves and the measured pH reaches 6.8.
- Bring the total solution to the selected final volume, mix thoroughly, and verify the final pH.
About This Buffer
PIPES is a zwitterionic biological buffer used in biochemical and cell-related applications in the mildly acidic to near-neutral pH region. This calculator scales a fixed 0.5 M PIPES free-acid stock formulation at pH 6.8, with NaOH added experimentally to reach the target pH.
Common questions
Which PIPES reagent form does this recipe use?
The preset uses PIPES free acid. The displayed mass should not be applied to a different salt form.
Why does the page not offer pH 6.5 or 7.0?
Only the reviewed 0.5 M PIPES-NaOH pH 6.8 formulation is currently published.
Why is the NaOH volume not calculated?
NaOH is used only to reach the measured pH. The calculator does not predict titrant volume because the actual requirement depends on preparation conditions.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
0.5 M PIPES-NaOH pH 6.8
Source location: Materials and recipes — exact 0.5 M PIPES-NaOH pH 6.8 composition; PIPES dissolution is facilitated by adding NaOH while mixing.
Preparation method: Source-stated quantitative composition
- Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A PMC9609470 · open article
- Purification of native Arp2/3 complex from bovine thymus PMC3994882 · open article
- A cell free system for functional centromere and kinetochore assembly PMC4011387 · open article