Sodium Acetate Buffer Calculator
Scale a fixed 0.1 M acetate buffer at pH 5.0 from a 0.2 M sodium acetate trihydrate stock and a 0.2 M acetic acid stock.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| 0.2 M sodium acetate stockprepared aqueous stock | 35.2 mLPrepared from sodium acetate trihydrate; theoretical 0.2 M basis is approximately 27.22 g/L. | |
| 0.2 M acetic acid stockprepared aqueous stock | 14.8 mL | |
| Purified water | Bring the total solution volume to 100 mL. Do not add 100 mL of water; add water only until the total solution reaches 100 mL. |
How to prepare 0.1 M acetate pH 5.0
- Measure the scaled volume of the defined 0.2 M sodium acetate stock prepared from sodium acetate trihydrate.
- Add the scaled volume of the defined 0.2 M acetic acid stock.
- Mix the two stocks with less than the selected final volume of purified water.
- Bring to the selected final volume, mix thoroughly, and verify the final pH experimentally.
About This Buffer
Sodium acetate and acetic acid form a commonly used buffering system for acidic laboratory solutions. Acetate buffers are used in a range of biochemical, molecular biology, and analytical procedures. This page provides one fixed 0.1 M acetate formulation at pH 5.0, prepared from a defined 0.2 M sodium acetate stock made from sodium acetate trihydrate and a 0.2 M acetic acid stock.
Common questions
Which acetate preset is publicly available?
Only the reviewed 0.1 M acetate pH 5.0 preset is published. It uses fixed volumes of two defined 0.2 M stocks.
Can I enter another acetate pH?
No. This page preserves the reviewed pH 5.0 stock ratio and does not interpolate or calculate another pH.
Does the calculator predict the final pH from the component ratio?
No. It scales the reviewed fixed stock ratio and instructs the user to verify the final pH experimentally.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
0.1 M acetate pH 5.0
Source location: Buffer formulation — exact pH 5.0 preparation from a fixed ratio of 0.2 M sodium acetate trihydrate stock and 0.2 M acetic acid stock.
Preparation method: Fixed mixing ratio of defined stock solutions
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