50X TAE Buffer Calculator
Scale a fixed 50X Tris-acetate-EDTA electrophoresis buffer recipe by final volume without adding an unsupported pH-adjustment step.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Tris basefree base | 242 g | |
| Glacial acetic acidglacial liquid | 57.1 mL | |
| 0.5 M EDTA solution, pH 8.0prepared aqueous stock | 100 mL | |
| Purified water | Bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
How to prepare 50X TAE
- Add the calculated Tris base to approximately 60–70% of the selected final volume of purified water and stir until dissolved.
- Add the scaled glacial acetic acid carefully while stirring.
- Add the calculated volume of 0.5 M EDTA solution, pH 8.0 and mix thoroughly.
- Bring to the selected final volume with purified water.
About This Buffer
TAE, or Tris-acetate-EDTA, is a widely used buffer system for agarose gel electrophoresis of nucleic acids. This calculator scales a fixed 50X TAE stock prepared from Tris base, glacial acetic acid, and a source-defined 0.5 M EDTA solution at pH 8.0. The reviewed 50X preset does not specify a separate final-stock pH or a separate pH-adjustment step.
Common questions
Is the 50X TAE stock pH adjusted?
Not in this fixed preset. The recipe is prepared from the listed components and brought to volume without a separate titration step.
How do I prepare 1X TAE from 50X stock?
Use a 1:50 dilution of the completed 50X stock when a protocol calls for 1X TAE.
Why is the EDTA stock form specified?
The recipe uses a source-defined 0.5 M EDTA solution at pH 8.0. The source does not establish a salt or hydration state, so the calculator does not infer one.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
50X TAE
Source location: Reagents — exact 50X TAE formulation using a source-defined 0.5 M EDTA solution at pH 8.0; the EDTA salt and hydration state are not specified.
Preparation method: Source-stated quantitative composition
- CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish PMC7757680 · open article