TBST Buffer Calculator
Scale fixed Tris-buffered saline with Tween 20 formulations at pH 7.4 or pH 7.6.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Reviewed 1X TBS pH 7.4 base buffersource-defined prepared buffer | Bring to the selected final volume with the same reviewed 1X TBS pH 7.4 base buffer. | |
| Tween 20neat liquid | 1 mL |
How to prepare TBST pH 7.4, 0.10% Tween 20
- Add less than the selected final volume of the 1X TBS pH 7.4 base buffer.
- Add the calculated Tween 20 for the fixed 0.10% preset.
- Mix gently until evenly dispersed, avoiding excessive foaming.
- Bring to the selected final volume with the same 1X TBS pH 7.4 base buffer, then mix thoroughly.
About This Buffer
TBST is Tris-buffered saline supplemented with Tween 20 and is commonly used as a wash buffer in western blotting and related immunoassays. This calculator preserves two source-specific formulations: pH 7.4 with 0.10% Tween 20 and pH 7.6 with 0.05% Tween 20. The TBS composition, pH handling, and Tween 20 concentration remain linked to the selected preset.
Common questions
Are the Tween 20 concentrations linked to the pH presets?
Yes. The reviewed pH 7.4 preset contains 0.10% Tween 20, while the pH 7.6 preset contains 0.05%.
Can I change the Tween 20 percentage independently?
No. Each published preset has a fixed TBS composition and Tween 20 concentration.
What is the difference between TBS and TBST?
TBST is a TBS formulation supplemented with Tween 20. The detergent amount is scaled with the final volume.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
TBST pH 7.4, 0.10% Tween 20
Source location: Reagents — reviewed 1X TBS pH 7.4 base buffer with 0.10% Tween 20; the base-buffer pH adjustment with 1 M HCl occurs in the referenced TBS preparation, not after Tween addition.
Preparation method: Reviewed base buffer plus a source-defined additive
- Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Respiratory Chain Complexes PMC7853962 · open article
View sources for all presets
TBST pH 7.6, 0.05% Tween 20
Source location: Solutions — 50 mM Tris, 150 mM NaCl, 0.05% Tween 20, pH 7.6; masses and Tween volume are calculated from the published concentrations and the adjustment reagent is not identified.
Preparation method: Mass derived from the published concentration
- Detecting reactive oxygen species by immunohistochemistry PMC4571457 · open article