TE Buffer Calculator, pH 8.0
Scale standard TE or low-EDTA TE at pH 8.0 from defined Tris-HCl and source-defined EDTA stocks.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| 1 M Tris-HCl pH 8.0prepared aqueous stock | 1 mL | |
| 0.5 M EDTA solution, pH 8.0prepared aqueous stock | 200 µL | |
| Purified water | Bring the total solution volume to 100 mL. Do not add 100 mL of water; add water only until the total solution reaches 100 mL. |
How to prepare Standard TE pH 8.0
- Select standard TE or low-EDTA TE and measure the calculated volume of 1 M Tris-HCl pH 8.0.
- Add the calculated volume of 0.5 M EDTA solution, pH 8.0 for the selected preset.
- Add purified water until the total solution reaches the selected final volume.
- Mix thoroughly.
About This Buffer
TE buffer combines Tris with EDTA and is widely used for handling and storage of DNA or RNA. Tris provides pH buffering, while EDTA chelates divalent metal ions. This page provides standard TE containing 10 mM Tris and 1 mM EDTA and a low-EDTA version containing 10 mM Tris and 0.1 mM EDTA, both based on defined pH 8.0 stock solutions.
Common questions
What is the difference between standard and low-EDTA TE?
Standard TE contains 1 mM EDTA, while the low-EDTA preset contains 0.1 mM EDTA. Both contain 10 mM Tris at pH 8.0.
Why is the EDTA stock specified at pH 8.0?
The preset is defined from a 0.5 M EDTA solution at pH 8.0. Because the source does not establish a salt or hydration state, the calculator represents it as a prepared aqueous stock without inferring one.
Does the calculator adjust the pH of the final TE buffer?
No separate titration is included. The selected recipe is assembled from defined pH 8.0 stocks and brought to final volume.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
Standard TE pH 8.0
Source location: Reagents — standard TE pH 8.0 made from defined Tris and source-defined EDTA stocks; the EDTA salt and hydration state are not specified.
Preparation method: Fixed mixing ratio of defined stock solutions
- A protocol for the detection of precision genome editing in human cells using One-pot DTECT PMC11422130 · open article
View sources for all presets
Low-EDTA TE pH 8.0
Source location: Reagents — source-defined final 10 mM Tris / 0.1 mM EDTA composition implemented with calculated volumes of the registered 1 M Tris-HCl and source-defined 0.5 M EDTA stocks; the EDTA salt and hydration state are not specified.
Preparation method: Source-defined final concentrations implemented with calculated volumes of defined stocks
- Rhizoctonia solani Infection Assay of Young Sugar Beet and Arabidopsis plantlets PMC8799671 · open article