0.1 M Tricine Buffer Calculator, pH 8.0
Scale a 0.1 M Tricine stock recipe at pH 8.0 using a transparent molecular-weight-based mass derivation and experimental NaOH adjustment.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Tricinefree compound | 17.92 gMass derived from 0.1 mol/L × 1 L × 179.17 g/mol. | |
| Sodium hydroxide | Adjust experimentally to pH 8.0. | |
| Purified water | After pH adjustment, bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
How to prepare 0.1 M Tricine pH 8.0
- Add the calculated Tricine mass to approximately 70–80% of the selected final volume of purified water.
- Stir until the Tricine dissolves completely.
- Add NaOH gradually while monitoring the pH until pH 8.0 is reached.
- Bring to the selected final volume, mix thoroughly, and verify the final pH.
About This Buffer
Tricine is a zwitterionic biological buffer used in a variety of biochemical applications and is also a component of specialized protein electrophoresis systems. This page is specifically for preparation of a standalone Tricine stock buffer. The calculator scales a fixed 0.1 M Tricine formulation at pH 8.0, with NaOH used experimentally for pH adjustment.
Common questions
How is the Tricine mass calculated?
The reviewed preset uses 0.1 M Tricine and a molecular weight of 179.17 g/mol. The reference mass is then scaled linearly by final volume.
Is the NaOH amount part of the calculated recipe?
No. The Tricine mass is quantitative, while NaOH remains an experimental pH-adjustment step.
Can I enter a different Tricine concentration?
No. This fixed-recipe page scales only the reviewed 0.1 M, pH 8.0 preset by final volume.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
0.1 M Tricine pH 8.0
Source location: Published use — 100 mM Tricine at pH 8.0; PubChem molecular weight; Dojindo GB19 preparation method — 17.917 g Tricine per liter and 0.1 M NaOH stock used to obtain the desired measured pH.
Preparation method: Mass derived from the published concentration
- In vitro and in vivo characterization of retinoid synthesis from beta-carotene PMC2587144 · open article
- Zinc coordination geometry and ligand binding affinity: structural and kinetic analysis PMC3119262 · open article
- PubChem Compound Summary for Tricine (CID 79784) PUBCHEM_TRICINE · public authority
- Dojindo Tricine (GB19) — Preparation Method DOJINDO_TRICINE_BUFFER · manufacturer protocol