Towbin Transfer Buffer Calculator
Scale a fixed Towbin transfer-buffer formulation made from 10X Tris-glycine transfer stock and 20% methanol.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| 10X Tris-glycine transfer bufferprepared aqueous stock | 100 mLThe source-defined stock contains 30.3 g Tris base and 144 g glycine per liter. | |
| Methanollaboratory-grade liquid | 200 mL | |
| Purified water | Bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
Do not routinely adjust the pH.
How to prepare Towbin transfer buffer with 20% methanol
- Measure the calculated volume of the defined 10X Tris-glycine transfer stock.
- Add purified water to less than the selected final volume and mix.
- Add the calculated methanol volume and mix thoroughly.
- Bring to the selected final volume with purified water. Do not add a separate pH-adjustment step.
About This Buffer
Towbin transfer buffer is widely used to transfer proteins from polyacrylamide gels to blotting membranes during western blotting. The conventional system contains Tris, glycine, and methanol. This calculator scales a source-defined formulation made from a 10X Tris-glycine transfer stock and 20% methanol, with a nominal pH of approximately 8.3 and no routine final-mixture pH adjustment specified.
Common questions
How much methanol is in this Towbin preset?
The reviewed formulation contains 20% v/v methanol.
What is the 10X transfer stock used by this recipe?
The defined stock contains Tris base and glycine at the source-specified 10X composition. The calculator scales its volume as one component.
Can I select a 10% methanol version?
Not on this page unless a separately sourced and reviewed 10% preset is added.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
Towbin transfer buffer with 20% methanol
Source location: PMC7953349: defined 10X Tris-glycine stock and 100 mL stock plus 200 mL methanol per liter preparation. Bio-Rad: standard Towbin final formulation of 25 mM Tris, 192 mM glycine, 20% methanol at approximately pH 8.3 without routine pH adjustment.
Preparation method: Fixed mixing ratio of defined stock solutions
- Efficient Transient Gene Knock-down in Tobacco Plants Using Carbon Nanocarriers PMC7953349 · open article
- Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell Instruction Manual BIO_RAD_TOWBIN_TRANSFER_BUFFER · manufacturer protocol