ChemHBuffer Calculator

10X Tris-Glycine Native Running Buffer Calculator

Scale a fixed 10X native PAGE running buffer containing Tris base and glycine without SDS.

Select a reviewed preset and enter the final volume to prepare.

Recipe for 1 L of 10X Tris-Glycine native running buffer
ReagentFormulaAmount or action
Tris basefree base30.3 g
Glycinefree compound144 g
Purified waterBring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L.
Nominal pH: approximately 8.3

How to prepare 10X Tris-Glycine native running buffer

  1. Add the calculated Tris base and glycine to approximately 70–80% of the selected final volume of purified water.
  2. Stir until both solids dissolve completely.
  3. Bring to the selected final volume with purified water and mix thoroughly.
  4. Do not add SDS to this native running-buffer stock.

About This Buffer

Tris-glycine native running buffer is used for non-denaturing protein electrophoresis in which SDS is intentionally omitted. Omitting SDS avoids the detergent-based denaturation produced by SDS, although preservation of native protein structure also depends on sample preparation and other electrophoresis conditions. This calculator scales a fixed 10X Tris-glycine formulation without SDS, with a nominal pH of approximately 8.3.

Common questions

Does this native running buffer contain SDS?

No. The reviewed native PAGE formulation contains Tris base and glycine only.

How do I prepare 1X native running buffer from 10X stock?

Use a 1:10 dilution of the completed 10X stock when the protocol calls for 1X buffer.

Can I convert the SDS running-buffer preset by simply removing SDS?

Use the dedicated native preset instead. It is maintained as a separately reviewed fixed formulation.

Sources and review notes

The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.

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