10X Tris-Glycine-SDS Running Buffer Calculator
Scale a fixed 10X SDS-PAGE running buffer recipe containing Tris base, glycine, and SDS with a nominal pH of approximately 8.3.
Select a reviewed preset and enter the final volume to prepare.
| Reagent | Formula | Amount or action |
|---|---|---|
| Tris basefree base | 30 g | |
| Glycinefree compound | 144 g | |
| Sodium dodecyl sulfateanhydrous | 10 g | |
| Purified water | Bring the total solution volume to 1 L. Do not add 1 L of water; add water only until the total solution reaches 1 L. |
Verify the final measured pH experimentally. Minimal or no pH adjustment is normally expected.
How to prepare 10X Tris-Glycine-SDS running buffer
- Add the calculated Tris base and glycine to approximately 70–80% of the selected final volume of purified water.
- Stir until the solids dissolve, then add the calculated SDS gradually and continue mixing until uniform.
- Bring to the selected final volume with purified water.
- Mix thoroughly, then check the measured pH. The expected pH is approximately 8.3; minimal or no adjustment is normally expected.
About This Buffer
Tris-glycine-SDS is a widely used running-buffer system for SDS-PAGE separation of proteins. Tris and glycine provide the principal buffer ions, while SDS helps maintain denaturing electrophoresis conditions. This calculator scales a fixed 10X Tris-glycine-SDS formulation with an expected pH of approximately 8.3. The measured pH should be checked after preparation; minimal or no adjustment is normally expected for this source-defined formulation, and no routine titrant is specified.
Common questions
Should I adjust the pH of Tris-glycine-SDS running buffer?
The expected pH is approximately 8.3. Check the measured pH after preparation; minimal or no adjustment is normally expected, and no routine titrant is specified.
How do I prepare 1X running buffer from 10X stock?
Use a 1:10 dilution of the completed 10X stock when a protocol calls for 1X running buffer.
What distinguishes this buffer from the native running buffer?
This formulation contains SDS. The native Tris-glycine preset omits SDS and must remain a separate recipe.
Sources and review notes
The source for the currently selected preset appears first. Recipe quantities remain tied to the cited article location.
10X Tris-Glycine-SDS running buffer
Source location: Recipes — source-defined 10X Tris-Glycine-SDS running buffer with expected pH approximately 8.3; check the measured pH after preparation, with minimal or no adjustment normally expected and no routine titrant specified.
Preparation method: Source-stated quantitative composition
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